Coding

Part:BBa_K3258023:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-20)


Dead Frameshift version of AcrIIA4 AntiCRISPR protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 244


Design Notes

Double frameshift designed to create nonfunctioning negative control for use with AcrIIA4 experiments.


Source

Synthetic part, derived from AcrIIA4 constructs from Dr. Stanley Qi's lab.

References